Ontology highlight
ABSTRACT:
SUBMITTER: Kim HJ
PROVIDER: S-EPMC5831329 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
Kim Hak Joong HJ Liu Yung-Nan YN McCarty Reid M RM Liu Hung-Wen HW
Journal of the American Chemical Society 20171107 45
Many cobalamin (Cbl)-dependent radical S-adenosyl-l-methionine (SAM) methyltransferases have been identified through sequence alignment and/or genetic analysis; however, few have been studied in vitro. GenK is one such enzyme that catalyzes methylation of the 6'-carbon of gentamicin X<sub>2</sub> (GenX<sub>2</sub>) to produce G418 during the biosynthesis of gentamicins. Reported herein, several alternative substrates and fluorinated substrate analogs were prepared to investigate the mechanism of ...[more]