Ontology highlight
ABSTRACT:
SUBMITTER: Schofield JA
PROVIDER: S-EPMC5831505 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Schofield Jeremy A JA Duffy Erin E EE Kiefer Lea L Sullivan Meaghan C MC Simon Matthew D MD
Nature methods 20180122 3
RNA sequencing (RNA-seq) offers a snapshot of cellular RNA populations, but not temporal information about the sequenced RNA. Here we report TimeLapse-seq, which uses oxidative-nucleophilic-aromatic substitution to convert 4-thiouridine into cytidine analogs, yielding apparent U-to-C mutations that mark new transcripts upon sequencing. TimeLapse-seq is a single-molecule approach that is adaptable to many applications and reveals RNA dynamics and induced differential expression concealed in tradi ...[more]