Ontology highlight
ABSTRACT:
SUBMITTER: Wu F
PROVIDER: S-EPMC5832616 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Wu Fuqing F Zhang Qi Q Wang Xiao X
Cell systems 20180207 2
Polycistronic architecture is common for synthetic gene circuits, however, it remains unknown how expression of one gene is affected by the presence of other genes/noncoding regions in the operon, termed adjacent transcriptional regions (ATR). Here, we constructed synthetic operons with a reporter gene flanked by different ATRs, and we found that ATRs with high GC content, small size, and low folding energy lead to high gene expression. Based on these results, we built a model of gene expression ...[more]