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Expression profiling across wild and cultivated tomatoes supports the relevance of early miR482/2118 suppression for Phytophthora resistance.


ABSTRACT: Plants possess a battery of specific pathogen resistance (R-)genes. Precise R-gene regulation is important in the presence and absence of a pathogen. Recently, a microRNA family, miR482/2118, was shown to regulate the expression of a major class of R-genes, nucleotide-binding site leucine-rich repeats (NBS-LRRs). Furthermore, RNA silencing suppressor proteins, secreted by pathogens, prevent the accumulation of miR482/2118, leading to an upregulation of R-genes. Despite this transcriptional release of R-genes, RNA silencing suppressors positively contribute to the virulence of some pathogens. To investigate this paradox, we analysed how the regulation of NBS-LRRs by miR482/2118 has been shaped by the coevolution between Phytophthora infestans and cultivated and wild tomatoes. We used degradome analyses and qRT-PCR to evaluate and quantify the co-expression of miR482/2118 and their NBS-LRR targets. Our data show that miR482/2118-mediated targeting contributes to the regulation of NBS-LRRs in Solanum lycopersicum. Based on miR482/2118 expression profiling in two additional tomato species-with different coevolutionary histories with P. infestans-we hypothesize that pathogen-mediated RNA silencing suppression is most effective in the interaction between S. lycopersicum and P. infestans Furthermore, an upregulation of miR482/2118 early in the infection may increase susceptibility to P. infestans.

SUBMITTER: de Vries S 

PROVIDER: S-EPMC5832704 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Expression profiling across wild and cultivated tomatoes supports the relevance of early miR482/2118 suppression for <i>Phytophthora</i> resistance.

de Vries Sophie S   Kukuk Andreas A   von Dahlen Janina K JK   Schnake Anika A   Kloesges Thorsten T   Rose Laura E LE  

Proceedings. Biological sciences 20180201 1873


Plants possess a battery of specific pathogen resistance (<i>R-</i>)genes. Precise <i>R-</i>gene regulation is important in the presence and absence of a pathogen. Recently, a microRNA family, miR482/2118, was shown to regulate the expression of a major class of <i>R-</i>genes<i>,</i> nucleotide-binding site leucine-rich repeats (<i>NBS-LRRs</i>). Furthermore, RNA silencing suppressor proteins, secreted by pathogens, prevent the accumulation of miR482/2118, leading to an upregulation of <i>R-</i  ...[more]

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