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Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1.


ABSTRACT: Synthetic peptides derived from ethylene-insensitive protein 2 (EIN2), a central regulator of ethylene signalling, were recently shown to delay fruit ripening by interrupting protein-protein interactions in the ethylene signalling pathway. Here, we show that the inhibitory peptide NOP-1 binds to the GAF domain of ETR1 - the prototype of the plant ethylene receptor family. Site-directed mutagenesis and computational studies reveal the peptide interaction site and a plausible molecular mechanism for the ripening inhibition.

SUBMITTER: Milic D 

PROVIDER: S-EPMC5832771 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Recognition motif and mechanism of ripening inhibitory peptides in plant hormone receptor ETR1.

Milić Dalibor D   Dick Markus M   Mulnaes Daniel D   Pfleger Christopher C   Kinnen Anna A   Gohlke Holger H   Groth Georg G  

Scientific reports 20180301 1


Synthetic peptides derived from ethylene-insensitive protein 2 (EIN2), a central regulator of ethylene signalling, were recently shown to delay fruit ripening by interrupting protein-protein interactions in the ethylene signalling pathway. Here, we show that the inhibitory peptide NOP-1 binds to the GAF domain of ETR1 - the prototype of the plant ethylene receptor family. Site-directed mutagenesis and computational studies reveal the peptide interaction site and a plausible molecular mechanism f  ...[more]

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