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A sleep state in Drosophila larvae required for neural stem cell proliferation.

ABSTRACT: Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larvae, but dopamine is not required. Using real-time behavioral monitoring in a closed-loop sleep deprivation system, we find that sleep loss in larvae impairs cell division of neural progenitors. This work establishes a system uniquely suited for studying sleep during nascent periods, and demonstrates that sleep in early life regulates neural stem cell proliferation.

SUBMITTER: Szuperak M

PROVIDER: S-EPMC5834245 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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A sleep state in Drosophila larvae required for neural stem cell proliferation.

Szuperak Milan M Churgin Matthew A MA Borja Austin J AJ Raizen David M DM Fang-Yen Christopher C Kayser Matthew S MS

eLife 20180209

Sleep during development is involved in refining brain circuitry, but a role for sleep in the earliest periods of nervous system elaboration, when neurons are first being born, has not been explored. Here we identify a sleep state in Drosophila larvae that coincides with a major wave of neurogenesis. Mechanisms controlling larval sleep are partially distinct from adult sleep: octopamine, the Drosophila analog of mammalian norepinephrine, is the major arousal neuromodulator in larva ...[more]

PMID: 29424688

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