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Bio-ethanol production from waste biomass of Pogonatherum crinitum phytoremediator: an eco-friendly strategy for renewable energy.

ABSTRACT: In this study, we have described three steps to produce ethanol from Pogonatherum crinitum, which was derived after the treatment of textile wastewater. (a) Production of biomass: biomass samples collected from a hydroponic P. crinitum phytoreactor treating dye textile effluents and augmented with Ca-alginate immobilized growth-promoting bacterium, Bacillus pumilus strain PgJ (consortium phytoreactor), and waste sorghum husks were collected and dried. Compositional analysis of biomass (consortium phytoreactor) showed that the concentration of cellulose, hemicelluloses and lignin was 42, 30 and 17%, respectively, whereas the biomass samples without the growth-promoting bacterium (normal phytoreactor) was slightly lower, 40, 29 and 16%, respectively. (b) Hydrolysate (sugar) production: a crude sample of the fungus, Phanerochaete chrysosporium containing hydrolytic enzymes such as endoglucanase (53.25 U/ml), exoglucanase (8.38 U/ml), glucoamylase (115.04 U/ml), xylanase (83.88 U/ml), LiP (0.972 U/ml) and MnP (0.459 U/ml) was obtained, and added to consortium, normal and control phytoreactor derived biomass supplemented with Tween-20 (0.2% v/v). The hydrolysate of biomass from consortium phytoreactor produced maximum reducing sugar (0.93 g/l) than hydrolysates of normal phytoreactor biomass (0.82 g/l) and control phytoreactor biomass (0.79 g/l). FTIR and XRD analysis confirmed structural changes in treated biomass. (c) Ethanol production: the bioethanol produced from enzymatic hydrolysates of waste biomass of consortium and normal phytoreactor using Saccharomyces cerevisiae (KCTC 7296) was 42.2 and 39.4 g/l, respectively, while control phytoreactor biomass hydrolysate showed only 25.5 g/l. Thus, the amalgamation of phytoremediation and bioethanol production can be the truly environment-friendly way to eliminate the problem of textile dye along with bioenergy generation.

SUBMITTER: Waghmare PR

PROVIDER: S-EPMC5834409 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Bio-ethanol production from waste biomass of Pogonatherum crinitum phytoremediator: an eco-friendly strategy for renewable energy.

Waghmare Pankajkumar R PR Watharkar Anuprita D AD Jeon Byong-Hun BH Govindwar Sanjay P SP

3 Biotech 20180302 3

In this study, we have described three steps to produce ethanol from Pogonatherum crinitum, which was derived after the treatment of textile wastewater. (a) Production of biomass: biomass samples collected from a hydroponic P. crinitum phytoreactor treating dye textile effluents and augmented with Ca-alginate immobilized growth-promoting bacterium, Bacillus pumilus strain PgJ (consortium phytoreactor), and waste sorghum husks were collected and dried. Compositional analysis ...[more]

PMID: 29515964

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Project description:Recent national strategic plans in China have set renewable energy targets for rural household energy programs, including those that advance the production of densified biomass fuels (e.g. pellets, briquettes) for use in household cooking and heating stoves. There is presently very little information on potential barriers to the successful development of densified biomass for household cooking and heating in China, but such knowledge may be informative in settings that aim to replace unprocessed coal and other polluting fuels with renewable, cleaner-burning energy sources. We designed a case study to coordinate data-gathering efforts at rural field sites in southwestern Sichuan province and northeastern Jilin and Heilongjiang provinces, where production of densified biomass fuels is under development for household end-users. We conducted interviews with factory personnel, local administrative leaders, and sector experts involved in the production and distribution of densified fuel, including pellets and briquettes, for household use. Results from our qualitative textual data analysis yielded several recommendations for improving development of densified biomass fuels for household end-use. These included reducing heterogeneity of feedstocks, increasing financial support for operational costs (e.g. collection, transport, and storage of raw materials; storage and distribution of final products), improving household perceptions of and subsequent demand for densified biomass fuels, and increasing enforcement of national and provincial policies banning the use of coal and open-field biomass burning. Collection and storage of raw materials and the final densified fuel product were consistently noted as critical challenges to scaling up production at all three sites. Finally, the perspectives of factory managers and local village administrators that we present also indicated that production of densified biomass fuels would most likely be more successful and reproducible in places where national-level policies are viewed as obligatory.

Project description:BackgroundPlant-associated microbes (endophytes) have a significant relationship to enhance plant growth and crop productivity by producing proficient bioactive metabolites. Since endophytes promoted plant growth either directly by releasing active metabolites such as phytohormones or indirectly by suppressing the growth of phytopathogens, so, in this work, biomass yield of local endophytic Trichoderma harzianum was maximized at shake-flask scale and scaled up via 7-L Bioflo310 fermenter using continuous exponential fed-batch fermentation mode. Subsequently, the effect of these cells as bio-fertilizer was assessed using two-barley grain genotypes (Russian and Egyptian seeds) via an intelligent hydroponic system based on Internet of Things (IoT).ResultsTo reduce the cost of a biomass production line, agro-waste media containing potato, onion, garlic, pea, and cabbage peels were chosen as the culturing medium. The pea peel medium was found to be the best producer of biomass (2.2 g/L). The cultivation factors were evaluated to improve this biomass yield. The results showed that the maximum biomass production (4.9 g/L) was reported by adjusting the medium pH at 5.0 that inoculated with 10% of spore suspension, then incubated at 30°C, and 200 rpm. Then, this biomass yield was scaled up kinetically (505.4 g/L) by using exponential fed-batch fermentation mode via a 7-L bioreactor. The stimulation impacts of this endophytic T. harzianum on the growth of different barley genotypes (Russian and Egyptian seeds) were determined using a controlled hydroponic chamber. The total chlorophyll, carotenoid, and carbohydrate amounts in treated Russian showed the proficient stimulation percentage (81.05, 80, 40.8%) compared to the Egyptian barley groups (76.39, 73.5, 25.9%) respectively. Also, the maximum carbohydrate content (83.95 ± 1.7%) was recorded in the case of Russian barley.ConclusionVia this work, the optimal combination conditions for the cost-effective biomass production of endophytic T. harzianum were designed industrially via a fed-batch fermentation system using the cheapest culturing medium. Furthermore, by applying this promising bio-fertilizer, the total cost of barley production via an IoT hydroponic growing system was reduced. Besides, these animal diets (sprouted barley) could be produced in 3 cycles per month.

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Bio-ethanol production from waste biomass of Pogonatherum crinitum phytoremediator: an eco-friendly strategy for renewable energy.

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Bio-ethanol production from waste biomass of <i>Pogonatherum crinitum</i> phytoremediator: an eco-friendly strategy for renewable energy.

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Bio-ethanol production from waste biomass of Pogonatherum crinitum phytoremediator: an eco-friendly strategy for renewable energy.

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Bio-ethanol production from waste biomass of &lt;i&gt;Pogonatherum crinitum&lt;/i&gt; phytoremediator: an eco-friendly strategy for renewable energy.

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OmicsDI is part of the ELIXIR infrastructure

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Bio-ethanol production from waste biomass of <i>Pogonatherum crinitum</i> phytoremediator: an eco-friendly strategy for renewable energy.