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Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.


ABSTRACT: DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of cis-2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen Pseudomonas aeruginosa (P. aeruginosa). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of dspI results in a remarkable decreased in the pyoverdine production, flagella-dependent swarming motility, and biofilm dispersion as well as attenuated virulence in P. aeruginosa PA14. This study thus unravels the mechanism of DspI in diffusible signal factor (DSF) CDA biosynthesis, providing vital information for developing inhibitors that interfere with DSF associated pathogenicity in P. aeruginosa.

SUBMITTER: Liu L 

PROVIDER: S-EPMC5834635 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.

Liu Li L   Li Tao T   Cheng Xing-Jun XJ   Peng Cui-Ting CT   Li Chang-Cheng CC   He Li-Hui LH   Ju Si-Min SM   Wang Ning-Yu NY   Ye Ting-Hong TH   Lian Mao M   Xiao Qing-Jie QJ   Song Ying-Jie YJ   Zhu Yi-Bo YB   Yu Luo-Ting LT   Wang Zhen-Ling ZL   Bao Rui R  

Scientific reports 20180302 1


DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of cis-2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen Pseudomonas aeruginosa (P. aeruginosa). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of  ...[more]

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