Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.
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ABSTRACT:
SUBMITTER: Liu L
PROVIDER: S-EPMC5834635 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
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Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.
Liu Li L Li Tao T Cheng Xing-Jun XJ Peng Cui-Ting CT Li Chang-Cheng CC He Li-Hui LH Ju Si-Min SM Wang Ning-Yu NY Ye Ting-Hong TH Lian Mao M Xiao Qing-Jie QJ Song Ying-Jie YJ Zhu Yi-Bo YB Yu Luo-Ting LT Wang Zhen-Ling ZL Bao Rui R
Scientific reports 20180302 1
DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of cis-2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen Pseudomonas aeruginosa (P. aeruginosa). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of ...[more]
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