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Fitness Advantage of mcr-1-Bearing IncI2 and IncX4 Plasmids in Vitro.


ABSTRACT: The objective of this study was to assess the impact of diverse plasmids bearing colistin resistance gene mcr-1 on host fitness. Forty-seven commensal E. coli isolates recovered from the pig farm where mcr-1 was first identified were screened for mcr-1. mcr-1-bearing plasmids were characterized by sequencing. The fitness impact of mcr-1-bearing plasmids was evaluated by in vitro competition assays. Twenty-seven (57.5%) E. coli isolates were positive for mcr-1. The mcr-1 genes were mainly located on plasmids belonging to IncI2 (n = 5), IncX4 (n = 11), IncHI2/ST3 (n = 8), IncFII (n = 2), and IncY (n = 2). InHI2 plasmids also carried other resistance genes (floR, blaCTX-M, and fosA3) and were only detected in isolates from nursery pigs. Sequences of the representative mcr-1-bearing plasmids were almost identical to those of the corresponding plasmid types reported previously. An increase in the fitness of IncI2- and IncX4-carrying strains was observed, while the presence of IncHI2, IncFII and IncY plasmids showed a fitness cost although an insignificant fitness increase was initially observed in IncFII or IncY plasmids-containing strains. Acquisition of IncI2-type plasmid was more beneficial for host E. coli DH5? than either IncHI2 or IncX4 plasmid, while transformants with IncHI2-type plasmid presented a competitive disadvantage against IncI2 or IncX4 plasmid containing strains. In conclusion, IncI2, IncX4, and IncHI2 were the major plasmid types driving the dissemination of mcr-1 in this farm. Increased fitness or co-selection by other antimicrobials might contribute to the further dissemination of the three epidemic mcr-1-positive plasmids (IncI2, IncX4, and IncHI2) in this farm and worldwide.

SUBMITTER: Wu R 

PROVIDER: S-EPMC5835064 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Fitness Advantage of <i>mcr-1</i>-Bearing IncI2 and IncX4 Plasmids <i>in Vitro</i>.

Wu Renjie R   Yi Ling-Xian LX   Yu Lin-Feng LF   Wang Jing J   Liu Yiyun Y   Chen Xiaojie X   Lv Luchao L   Yang Jun J   Liu Jian-Hua JH  

Frontiers in microbiology 20180227


The objective of this study was to assess the impact of diverse plasmids bearing colistin resistance gene <i>mcr-1</i> on host fitness. Forty-seven commensal <i>E. coli</i> isolates recovered from the pig farm where <i>mcr-1</i> was first identified were screened for <i>mcr-1. mcr-1</i>-bearing plasmids were characterized by sequencing. The fitness impact of <i>mcr-1</i>-bearing plasmids was evaluated by <i>in vitro</i> competition assays. Twenty-seven (57.5%) <i>E. coli</i> isolates were positi  ...[more]

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