Ontology highlight
ABSTRACT:
SUBMITTER: Ko CW
PROVIDER: S-EPMC5836109 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Ko Chih-Wei CW Counihan Daniel D Wu Jing J Hatzoglou Maria M Puchowicz Michelle A MA Croniger Colleen M CM
The Journal of biological chemistry 20180109 9
Phosphoenolpyruvate carboxykinase (Pck1) is a metabolic enzyme that is integral to the gluconeogenic and glyceroneogenic pathways. However, Pck1's role in macrophage metabolism and function is unknown. Using stable isotopomer MS analysis in a mouse model with a myeloid cell-specific <i>Pck1</i> deletion, we show here that this deletion increases the proinflammatory phenotype in macrophages. Incubation of LPS-stimulated bone marrow-derived macrophages (BMDM) with [U-<sup>13</sup>C]glucose reveale ...[more]