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RNAseq analysis of bronchial epithelial cells to identify COPD-associated genes and SNPs.

ABSTRACT: BACKGROUND:There is a need for more powerful methods to identify low-effect SNPs that contribute to hereditary COPD pathogenesis. We hypothesized that SNPs contributing to COPD risk through cis-regulatory effects are enriched in genes comprised by bronchial epithelial cell (BEC) expression patterns associated with COPD. METHODS:To test this hypothesis, normal BEC specimens were obtained by bronchoscopy from 60 subjects: 30 subjects with COPD defined by spirometry (FEV1/FVC

SUBMITTER: Yeo J

PROVIDER: S-EPMC5838965 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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RNAseq analysis of bronchial epithelial cells to identify COPD-associated genes and SNPs.

Yeo Jiyoun J Morales Diego A DA Chen Tian T Crawford Erin L EL Zhang Xiaolu X Blomquist Thomas M TM Levin Albert M AM Massion Pierre P PP Arenberg Douglas A DA Midthun David E DE Mazzone Peter J PJ Nathan Steven D SD Wainz Ronald J RJ Nana-Sinkam Patrick P Willey Paige F S PFS Arend Taylor J TJ Padda Karanbir K Qiu Shuhao S Federov Alexei A Hernandez Dawn-Alita R DR Hammersley Jeffrey R JR Yoon Youngsook Y Safi Fadi F Khuder Sadik A SA Willey James C JC

BMC pulmonary medicine 20180305 1

<h4>Background</h4>There is a need for more powerful methods to identify low-effect SNPs that contribute to hereditary COPD pathogenesis. We hypothesized that SNPs contributing to COPD risk through cis-regulatory effects are enriched in genes comprised by bronchial epithelial cell (BEC) expression patterns associated with COPD.<h4>Methods</h4>To test this hypothesis, normal BEC specimens were obtained by bronchoscopy from 60 subjects: 30 subjects with COPD defined by spirometry (FEV1/FVC < 0.7, ...[more]

PMID: 29506519

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