Comparison of two insulin assays for first-phase insulin release in type 1 diabetes prediction and prevention studies.

ABSTRACT:

Background

Detection of below-threshold first-phase insulin release or FPIR (1+3 minute insulin concentrations during an intravenous glucose tolerance test [IVGTT]) is important in type 1 diabetes prediction and prevention studies including the TrialNet Oral Insulin Prevention Trial. We assessed whether an insulin immunoenzymometric assay (IEMA) could replace the less practical but current standard of a radioimmunoassay (RIA) for FPIR.

Methods

One hundred thirty-three islet autoantibody positive relatives of persons with type 1 diabetes underwent 161 IVGTTs. Insulin concentrations were measured by both assays in 1056 paired samples. A rule classifying FPIR (below-threshold, above-threshold, uncertain) by the IEMA was derived and validated against FPIR by the RIA.

Results<

SUBMITTER: Mahon JL 

PROVIDER: S-EPMC5839139 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature