Neutrophil-generated HOCl leads to non-specific thiol oxidation in phagocytized bacteria.
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ABSTRACT:
SUBMITTER: Degrossoli A
PROVIDER: S-EPMC5839695 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Publications
Neutrophil-generated HOCl leads to non-specific thiol oxidation in phagocytized bacteria.
Degrossoli Adriana A Müller Alexandra A Xie Kaibo K Schneider Jannis F JF Bader Verian V Winklhofer Konstanze F KF Meyer Andreas J AJ Leichert Lars I LI
eLife 20180306
Phagocytic immune cells kill pathogens in the phagolysosomal compartment with a cocktail of antimicrobial agents. Chief among them are reactive species produced in the so-called oxidative burst. Here, we show that bacteria exposed to a neutrophil-like cell line experience a rapid and massive oxidation of cytosolic thiols. Using roGFP2-based fusion probes, we could show that this massive breakdown of the thiol redox homeostasis was dependent on phagocytosis, presence of NADPH oxidase and ultimate ...[more]
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