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Open-source recombinant monoclonal secondary nanobodies.


ABSTRACT: Secondary antibodies are everyday reagents in biomedical research that are generated in animals. In this issue, Pleiner et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201709115) describe several single domain antibody fragments against antibodies from mouse and rabbit, so-called nanobodies that are easily produced recombinantly, and characterize their use in Western blotting, enzyme-linked immunosorbent assay, and immunofluorescence assays.

SUBMITTER: Ewers H 

PROVIDER: S-EPMC5839800 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Open-source recombinant monoclonal secondary nanobodies.

Ewers Helge H  

The Journal of cell biology 20180214 3


Secondary antibodies are everyday reagents in biomedical research that are generated in animals. In this issue, Pleiner et al. (2018. <i>J. Cell Biol.</i> https://doi.org/10.1083/jcb.201709115) describe several single domain antibody fragments against antibodies from mouse and rabbit, so-called nanobodies that are easily produced recombinantly, and characterize their use in Western blotting, enzyme-linked immunosorbent assay, and immunofluorescence assays. ...[more]

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