Differential Phagocytic Properties of CD45^low Microglia and CD45^high Brain Mononuclear Phagocytes-Activation and Age-Related Effects.

ABSTRACT: In the central nervous system (CNS), microglia are innate immune mononuclear phagocytes (CNS MPs) that can phagocytose infectious particles, apoptotic cells, neurons, and pathological protein aggregates, such as Aβ in Alzheimer's disease (AD). While CD11b⁺CD45^low microglia account for the majority of CNS MPs, a small population of CD11b⁺CD45^high CNS MPs is also recognized in AD that surround Aβ plaques. These transcriptionally and pathologically unique CD45^high cells have unclear origin and undefined phagocytic characteristics. We have comprehensively validated rapid flow cytometric assays of bulk-phase and amyloid β fibril (fAβ) phagocytosis and applied these to study acutely isolated CNS MPs. Using these methods, we provide novel ins

SUBMITTER: Rangaraju S 

PROVIDER: S-EPMC5840283 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature