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Photosystem II Subunit S overexpression increases the efficiency of water use in a field-grown crop.

ABSTRACT: Insufficient water availability for crop production is a mounting barrier to achieving the 70% increase in food production that will be needed by 2050. One solution is to develop crops that require less water per unit mass of production. Water vapor transpires from leaves through stomata, which also facilitate the influx of CO2 during photosynthetic assimilation. Here, we hypothesize that Photosystem II Subunit S (PsbS) expression affects a chloroplast-derived signal for stomatal opening in response to light, which can be used to improve water-use efficiency. Transgenic tobacco plants with a range of PsbS expression, from undetectable to 3.7 times wild-type are generated. Plants with increased PsbS expression show less stomatal opening in response to light, resulting in a 25% reduction in water loss per CO2 assimilated under field conditions. Since the role of PsbS is universal across higher plants, this manipulation should be effective across all crops.

SUBMITTER: Glowacka K

PROVIDER: S-EPMC5840416 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Photosystem II Subunit S overexpression increases the efficiency of water use in a field-grown crop.

Głowacka Katarzyna K Kromdijk Johannes J Kucera Katherine K Xie Jiayang J Cavanagh Amanda P AP Leonelli Lauriebeth L Leakey Andrew D B ADB Ort Donald R DR Niyogi Krishna K KK Long Stephen P SP

Nature communications 20180306 1

Insufficient water availability for crop production is a mounting barrier to achieving the 70% increase in food production that will be needed by 2050. One solution is to develop crops that require less water per unit mass of production. Water vapor transpires from leaves through stomata, which also facilitate the influx of CO<sub>2</sub> during photosynthetic assimilation. Here, we hypothesize that Photosystem II Subunit S (PsbS) expression affects a chloroplast-derived signal for stomatal open ...[more]

PMID: 29511193

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Project description:Highlights • We derive a model that provides an exact solution to the substrate-water exchange kinetics in a double-conformation system.• This model is used to interpret recently published data for Ca2+- and Sr2+-containing PSII in the S2 state, in which the g = 2.0 and g = 4.1 conformations coexist.• The component concentrations derived from the kinetic model provide an analytic description of the substrate-water exchange kinetics.• Contrary to previous reports, there is no significant effect of substituting Sr2+ for Ca2+ on any of the exchange rate constants.• The exchange rate of the slowly-exchanging water (Ws) in the S2 state g = 4.1 conformation is much faster than that in the g = 2.0 conformation, consistent with the assignment of Ws to W1 or W2 bound as terminal ligands to Mn4. We derive a model that provides an exact solution to the substrate-water exchange kinetics in a double-conformation system and use this model to interpret recently published data for Ca2+- and Sr2+-containing PSII in the S2 state, in which the g = 2.0 and g = 4.1 conformations coexist. The component concentrations derived from the kinetic model provide an analytic description of the substrate-water exchange kinetics, allowing us to more accurately interpret the results. Based on this model and the previously reported data on the S2 state g = 2.0 conformation, we obtain the substrate-water exchange rates of the g = 4.1 conformation and the conformational change rates. Two conclusions are made from the analyses. First, contrary to previous reports, there is no significant effect of substituting Sr2+ for Ca2+ on any of the exchange rate constants. Second, the exchange rate of the slowly-exchanging water (Ws) in the S2 state g = 4.1 conformation is much faster than that in the S2 state g = 2.0 conformation. The second conclusion is consistent with the assignment of Ws to W1 or W2 bound as terminal ligands to Mn4; Mn4 has been proposed to undergo an oxidation state change from Mn(IV) in the g = 2.0 conformation to Mn(III) in the g = 4.1 conformation.

Dataset Information

Photosystem II Subunit S overexpression increases the efficiency of water use in a field-grown crop.

Publications

Photosystem II Subunit S overexpression increases the efficiency of water use in a field-grown crop.

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