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Effects of xylo-oligosaccharide and flavomycin on the immune function of broiler chickens.

ABSTRACT: This study investigated the effects of xylo-oligosaccharide (XOS) and flavomycin (FLA) on the performance and immune function of broiler chickens. A total of 150 ArborAcres broilers were randomly divided into three groups and fed for six weeks from one day of age in cascade cages. The diets of each test group were (1) a basal diet, (2) the basal diet supplemented with 2 mg/kg FLA, and (3) the basal diet supplemented with 2 mg/kg XOS. At 21 and 42 days, the growth performance index values and short-chain fatty acid (SCFA) concentrations in the cecum were quantified. Furthermore, immunoglobulin G (IgG) and plasma interleukin 2 (IL-2) as well as mRNA expression of LPS-Induced TNF-alpha Factor (LITAF), Toll-like receptor-5 (TLR5) and interferon gamma (IFN? ) in the jejunum were quantified. The results showed that administration of XOS or FLA to chickens significantly improved the average daily gain. Supplementation with XOS increased acetate and butyrate in the cecum, while FLA supplementation increased propionate in the cecum. An increase in plasma IgG was observed in XOS-fed 21-day-old broilers, but FLA supplementation decreased IgG in the plasma of 42-day-old broilers and increased plasma IL-2. Furthermore, FLA or XOS supplementation downregulated mRNA expression of IFN? , LITAF and TLR5. The above data suggest that addition of XOS and FLA to the diet could improve the growth performance of broilers and reduce the expression of cytokine genes by stimulating SCFA.

SUBMITTER: Yuan L

PROVIDER: S-EPMC5842763 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Effects of xylo-oligosaccharide and flavomycin on the immune function of broiler chickens.

Yuan Lin L Li Wanli W Huo Qianqian Q Du Chenhong C Wang Zhixiang Z Yi Baodi B Wang Mingfa M

PeerJ 20180305

This study investigated the effects of xylo-oligosaccharide (XOS) and flavomycin (FLA) on the performance and immune function of broiler chickens. A total of 150 ArborAcres broilers were randomly divided into three groups and fed for six weeks from one day of age in cascade cages. The diets of each test group were (1) a basal diet, (2) the basal diet supplemented with 2 mg/kg FLA, and (3) the basal diet supplemented with 2 mg/kg XOS. At 21 and 42 days, the growth performance index values and sho ...[more]

PMID: 29527412

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Project description:Simple Summary The study was conducted to explore effects of xylose with different polymerizations on growth performance, intestinal barrier function, and gut microbial composition in weaned piglets. Results showed that dietary supplementation of 1% xylo-oligosaccharide (XOS) or 1% arabinoxylan (AX) reduced diarrhea incidence of piglets compared with the control group (CON) but increased intestinal villus height, antioxidase activity, and sIgA contents (p < 0.05). With in vitro fermentation assay, XOS showed a sharper reduction of the pH curve than AX (p < 0.05). XOS increased the abundance of Lactobacillus and Bifidobacterium in the ileal digesta (p < 0.05), while AX increased the abundance of Bifidobacterium in the colonic digesta (p < 0.05). In conclusion, both XOS and AX improved the intestinal barrier function and reduced the diarrhea incidence of weaned piglets, and the XOS showed a faster microbial fermentation than the AX due to a lower polymerization and molecular mass. Abstract The purpose of this study was to explore the effects of xylose with different polymerizations on growth performance, intestinal barrier function, and gut microbial composition in weaned piglets. A total of 144 weaned piglets were assigned to 3 dietary treatments in a completely randomized design according to their body weight and sex. Dietary treatments included a corn-soybean meal basal diet (CON) and 2 additional diets formulated with 1% arabinoxylan (AX) and 1% xylo-oligosaccharide (XOS), respectively. Results showed that dietary supplementation of XOS or AX reduced diarrhea incidence of weaned piglets compared with the CON group (p < 0.05). XOS or AX increased the ileal villus height and intestinal activity of antioxidases in weaned piglets compared with the CON group (p < 0.05). XOS or AX reduced the ileal and colonic IL-6 content and increased the colonic sIgA and IL-10 concentrations in weaned piglets compared with the CON group (p < 0.05). XOS or AX increased the total organic acids concentrations in the ileum and in vitro fermentation (p < 0.05). XOS increased the abundance of Lactobacillus and Bifidobacterium in the ileal digesta (p < 0.05), while AX increased the population of Lactobacillus in the ileal digesta and the abundance of Bifidobacterium in the colonic digesta of weaned piglets (p < 0.05). In conclusion, both XOS and AX reduce diarrhea incidence and improve antioxidant capacity, immune function, and populations of beneficial bacteria, while microbial fermentation of XOS with a lower polymerization and molecular mass can produce more organic acids and an increased abundance of Lactobacillus and Bifidobacterium in the upper gut of weaned pigs compared with AX.

Project description:Gut microbiota dysbiosis is closely associated with ulcerative colitis (UC). Prebiotic therapy is a potential approach for UC management especially remission maintaining. Xylo-oligosaccharide (XOS) is an efficient prebiotic with proven health benefits and few side effects. However, the effects of XOS on the gut microbiota of patients with UC have not been investigated previously. The aim of this study was to evaluate the prebiotic effects of XOS on the fecal microbiota of patients with UC in clinical remission using an in vitro fermentation model. Five patients with UC in clinical remission and five healthy volunteers were enrolled in this study. Fresh fecal samples of UC patients were diluted and inoculated in yeast extract, casitone and fatty acid (YCFA) medium alone or with XOS. After fermentation for 48 h, samples were collected for 16S rDNA sequencing to investigate the gut microbiota composition. Differences in the gut microbiota between healthy volunteers and UC patients in clinical remission were detected using original fecal samples. Subsequently, the differences between the YCFA medium alone or with XOS samples were analyzed to illustrate the effects of XOS on the gut microbiota of UC patients. In both principal coordinate analysis (PCoA) and principal component analysis (PCA), the fecal samples of UC patients differed from those of healthy volunteers. Linear discriminant analysis effect size (LEfSe) analysis revealed that the relative abundances of g_Roseburia and g_Lachnospiraceae_ND3007_group were higher in healthy volunteers than in UC patients, while o_Lactobacillales abundance showed the opposite trend (P < 0.05). Wilcoxon rank-sum test bar plot showed that the abundances of g_Eubacterium_halli_group and g_Lachnospiraceae_ND3007_group were higher in the healthy volunteers than in the UC patients (P < 0.05). In addition, in UC patients, the Wilcoxon rank-sum test showed that XOS fermentation promoted the growth of bacterial groups including g_Roseburia, g_Bifidobacterium, and g_Lactobacillus, which is beneficial for recovery of intestinal diseases. These results suggest that XOS can relieve dysbiosis in the feces of UC patients in clinical remission and thus represent a potential prebiotic material for maintaining remission.

Project description:This study evaluated the effects of dietary anticoccidial drugs plus antibiotic growth promoters (AGPs) on parameters of immunity in commercial broiler chickens. Day-old chicks were raised on used litter from a farm with endemic gangrenous dermatitis to simulate natural pathogen exposure and provided with diets containing decoquinate (DECX) or monensin (COBN) as anticoccidials plus bacitracin methylene disalicylate and roxarsone as AGPs. As a negative control, the chickens were fed with a non-supplemented diet. Immune parameters examined were concanavalin A (ConA)-stimulated spleen cell proliferation, intestine intraepithelial lymphocyte (IEL) and spleen cell subpopulations, and cytokine/chemokine mRNA levels in IELs and spleen cells. ConA-induced proliferation was decreased at 14 d post-hatch in DECX-treated chickens, and increased at 25 and 43 d in COBN-treated animals, compared with untreated controls. In DECX-treated birds, increased percentages of MHC2(+) and CD4(+) IELS were detected at 14 d, but decreased percentages of these cells were seen at 43 d, compared with untreated controls, while increased TCR2(+) IELs were evident at the latter time. Dietary COBN was associated with decreased fractions of MHC2(+) and CD4(+) IELs and reduced percentages of MHC2(+), BU1(+), and TCR1(+) spleen cells compared with controls. The levels of transcripts for interleukin-4 (IL-4), IL-6, IL-17F, IL-13, CXCLi2, interferon-γ (IFN-γ), and transforming growth factorβ4 were elevated in IELs, and those for IL-13, IL-17D, CXCLi2, and IFN-γ were increased in spleen cells, of DECX- and/or COBN-treated chickens compared with untreated controls. By contrast, IL-2 and IL-12 mRNAs in IELs, and IL-4, IL-12, and IL-17F transcripts in spleen cells, were decreased in DECX- and/or COBN-treated chickens compared with controls. These results suggest that DECX or COBN, in combination with bacitracin and roxarsone, modulate the development of the chicken post-hatch immune system.

Dataset Information

Effects of xylo-oligosaccharide and flavomycin on the immune function of broiler chickens.

Publications

Effects of xylo-oligosaccharide and flavomycin on the immune function of broiler chickens.

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