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Towards Discovery and Targeted Peptide Biomarker Detection Using nanoESI-TIMS-TOF MS.


ABSTRACT: In the present work, the potential of trapped ion mobility spectrometry coupled to TOF mass spectrometry (TIMS-TOF MS) for discovery and targeted monitoring of peptide biomarkers from human-in-mouse xenograft tumor tissue was evaluated. In particular, a TIMS-MS workflow was developed for the detection and quantification of peptide biomarkers using internal heavy analogs, taking advantage of the high mobility resolution (R = 150-250) prior to mass analysis. Five peptide biomarkers were separated, identified, and quantified using offline nanoESI-TIMS-CID-TOF MS; the results were in good agreement with measurements using a traditional LC-ESI-MS/MS proteomics workflow. The TIMS-TOF MS analysis permitted peptide biomarker detection based on accurate mobility, mass measurements, and high sequence coverage for concentrations in the 10-200 nM range, while simultaneously achieving discovery measurements of not initially targeted peptides as markers from the same proteins and, eventually, other proteins. Graphical Abstract ?.

SUBMITTER: Garabedian A 

PROVIDER: S-EPMC5844780 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Towards Discovery and Targeted Peptide Biomarker Detection Using nanoESI-TIMS-TOF MS.

Garabedian Alyssa A   Benigni Paolo P   Ramirez Cesar E CE   Baker Erin S ES   Liu Tao T   Smith Richard D RD   Fernandez-Lima Francisco F  

Journal of the American Society for Mass Spectrometry 20170909 5


In the present work, the potential of trapped ion mobility spectrometry coupled to TOF mass spectrometry (TIMS-TOF MS) for discovery and targeted monitoring of peptide biomarkers from human-in-mouse xenograft tumor tissue was evaluated. In particular, a TIMS-MS workflow was developed for the detection and quantification of peptide biomarkers using internal heavy analogs, taking advantage of the high mobility resolution (R = 150-250) prior to mass analysis. Five peptide biomarkers were separated,  ...[more]

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