Ontology highlight
ABSTRACT:
SUBMITTER: Long CP
PROVIDER: S-EPMC5845446 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Long Christopher P CP Gonzalez Jacqueline E JE Sandoval Nicholas R NR Antoniewicz Maciek R MR
Metabolic engineering 20160519
Understanding the impact of gene knockouts on cellular physiology, and metabolism in particular, is centrally important to quantitative systems biology and metabolic engineering. Here, we present a comprehensive physiological characterization of wild-type Escherichia coli and 22 knockouts of enzymes in the upper part of central carbon metabolism, including the PTS system, glycolysis, pentose phosphate pathway and Entner-Doudoroff pathway. Our results reveal significant metabolic changes that are ...[more]