Ontology highlight
ABSTRACT:
SUBMITTER: Walters CR
PROVIDER: S-EPMC5845795 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Chemical communications (Cambridge, England) 20180201 14
Thioamide substitutions in the backbones of proteins can modulate their structure and thermostability, or serve as spectroscopic probes in fluorescence quenching experiments. Using native chemical ligation, we have produced the first examples of a protein (calmodulin) containing two thioamides. Dithioamide variants were made to explore the effects of combining stabilizing, neutral, and destabilizing single thioamide substitutions. One of the dithioamide calmodulin variants exhibited stabilizatio ...[more]