Ontology highlight
ABSTRACT:
SUBMITTER: Gregory DJ
PROVIDER: S-EPMC5845820 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Gregory David J DJ Zhang Yiming Y Kobzik Lester L Fedulov Alexey V AV
Epigenetics 20130905 11
The ability to specifically reactivate epigenetically silenced genes would have great utility in experimental studies and potential therapeutic value. Here, we describe the specific targeting of thymidine DNA glycosylase (TDG), an enzyme involved in the mechanism of methylcytosine demethylation, to the promoter of Nos2, a gene silenced by methylation in fibroblasts, using artificial zinc finger DNA binding domains. Individual targeted TDG constructs had a small effect on Nos2 expression and meth ...[more]