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An Inexpensive, In-House-Made, Microdialysis Device for Measuring Drug-Protein Binding.

ABSTRACT: An inexpensive, in-house made microdialysis device is described that is suitable for measuring the binding of small molecules including drug candidates to serum proteins or other macromolecules. The device is based on the standard equilibrium dialysis method to measure the fraction of low molecular weight compound bound to proteins. It is constructed from a standard polypropylene 96-well plate, dialysis tubing, and low viscosity epoxy resin. The device can be readily prepared for a small fraction of the cost of a commercial, multichamber microdialysis device. Drug-protein binding results are provided, which validates the device.

SUBMITTER: Herbst ZM

PROVIDER: S-EPMC5846030 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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An Inexpensive, In-House-Made, Microdialysis Device for Measuring Drug-Protein Binding.

Herbst Zackary M ZM Shibata Sayaka S Fan Erkang E Gelb Michael H MH

ACS medicinal chemistry letters 20170928 3

An inexpensive, in-house made microdialysis device is described that is suitable for measuring the binding of small molecules including drug candidates to serum proteins or other macromolecules. The device is based on the standard equilibrium dialysis method to measure the fraction of low molecular weight compound bound to proteins. It is constructed from a standard polypropylene 96-well plate, dialysis tubing, and low viscosity epoxy resin. The device can be readily prepared for a small fractio ...[more]

PMID: 29541374

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