Dataset Information

Reactive oxygen species, nitric oxide production and antioxidant gene expression during development of aerenchyma formation in wheat.

ABSTRACT: In response to hypoxia, plant roots produce very high levels of nitric oxide. Recently, it was demonstrated that NO and ethylene both are essential for development of aerenchyma in wheat roots under hypoxia. Increased NO under hypoxia correlated with induction of NADPH oxidase gene expression, ROS production and lipid peroxidation in cortical cells. Tyrosine nitration was prominent in cells developing aerenchyma suggesting that NO and ROS play a key role in development of aerenchyma. However, the role of antioxidant genes during development of aerenchyma is not known, therefore, we checked gene expression of various antioxidants such as SOD1, AOX1A, APX and MnSOD at different time points after hypoxia treatment and found that expression of these genes elevated in 2 h but downregulated in 24 h where development of aerenchyma is prominent. Further, we found that plants growing under ammonium nutrition displayed delayed aerenchyma development. Taken together, new insights presented in this short communication highlighted additional regulatory role of antioxidants gene expression during aerenchyma development.

SUBMITTER: Wany A

PROVIDER: S-EPMC5846502 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Reactive oxygen species, nitric oxide production and antioxidant gene expression during development of aerenchyma formation in wheat.

Wany Aakanksha A Gupta Kapuganti Jagadis KJ

Plant signaling & behavior 20180206 2

In response to hypoxia, plant roots produce very high levels of nitric oxide. Recently, it was demonstrated that NO and ethylene both are essential for development of aerenchyma in wheat roots under hypoxia. Increased NO under hypoxia correlated with induction of NADPH oxidase gene expression, ROS production and lipid peroxidation in cortical cells. Tyrosine nitration was prominent in cells developing aerenchyma suggesting that NO and ROS play a key role in development of aerenchyma. However, th ...[more]

PMID: 29336716

Similar Datasets

Project description:It is now well accepted that radiation induced bystander effects can occur in cells exposed to media from irradiated cells. The aim of this study was to follow the bystander cells in real time following addition of media from irradiated cells and to determine the effect of inhibiting these signals. A human keratinocyte cell line, HaCaT cells, was irradiated (0.005, 0.05 and 0.5 Gy) with γ irradiation, conditioned medium was harvested after one hour and added to recipient bystander cells. Reactive oxygen species, nitric oxide, Glutathione levels, caspase activation, cytotoxicity and cell viability was measured after the addition of irradiated cell conditioned media to bystander cells. Reactive oxygen species and nitric oxide levels in bystander cells treated with 0.5Gy ICCM were analysed in real time using time lapse fluorescence microscopy. The levels of reactive oxygen species were also measured in real time after the addition of extracellular signal-regulated kinase and c-Jun amino-terminal kinase pathway inhibitors. ROS and glutathione levels were observed to increase after the addition of irradiated cell conditioned media (0.005, 0.05 and 0.5 Gy ICCM). Caspase activation was found to increase 4 hours after irradiated cell conditioned media treatment (0.005, 0.05 and 0.5 Gy ICCM) and this increase was observed up to 8 hours and there after a reduction in caspase activation was observed. A decrease in cell viability was observed but no major change in cytotoxicity was found in HaCaT cells after treatment with irradiated cell conditioned media (0.005, 0.05 and 0.5 Gy ICCM). This study involved the identification of key signaling molecules such as reactive oxygen species, nitric oxide, glutathione and caspases generated in bystander cells. These results suggest a clear connection between reactive oxygen species and cell survival pathways with persistent production of reactive oxygen species and nitric oxide in bystander cells following exposure to irradiated cell conditioned media.

Dataset Information

Reactive oxygen species, nitric oxide production and antioxidant gene expression during development of aerenchyma formation in wheat.

Publications

Reactive oxygen species, nitric oxide production and antioxidant gene expression during development of aerenchyma formation in wheat.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets