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3D second harmonic generation imaging tomography by multi-view excitation.

ABSTRACT: Biological tissues have complex 3D collagen fiber architecture that cannot be fully visualized by conventional second harmonic generation (SHG) microscopy due to electric dipole considerations. We have developed a multi-view SHG imaging platform that successfully visualizes all orientations of collagen fibers. This is achieved by rotating tissues relative to the excitation laser plane of incidence, where the complete fibrillar structure is then visualized following registration and reconstruction. We evaluated high frequency and Gaussian weighted fusion reconstruction algorithms, and found the former approach performs better in terms of the resulting resolution. The new approach is a first step toward SHG tomography.

SUBMITTER: Campbell KR

PROVIDER: S-EPMC5847324 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature

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3D second harmonic generation imaging tomography by multi-view excitation.

Campbell Kirby R KR Wen Bruce B Shelton Emily M EM Swader Robert R Cox Benjamin L BL Eliceiri Kevin K Campagnola Paul J PJ

Optica 20170928 10

Biological tissues have complex 3D collagen fiber architecture that cannot be fully visualized by conventional second harmonic generation (SHG) microscopy due to electric dipole considerations. We have developed a multi-view SHG imaging platform that successfully visualizes all orientations of collagen fibers. This is achieved by rotating tissues relative to the excitation laser plane of incidence, where the complete fibrillar structure is then visualized following registration and reconstructio ...[more]

PMID: 29541654

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