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Building the atomic model of a boreal lake virus of unknown fold in a 3.9?A cryo-EM map.

ABSTRACT: We report here the protocol adopted to build the atomic model of the newly discovered virus FLiP (Flavobacterium infecting, lipid-containing phage) into 3.9?Å cryo-electron microscopy (cryo-EM) maps. In particular, this report discusses the combination of density modification procedures, automatic model building and bioinformatics tools applied to guide the tracing of the major capsid protein (MCP) of this virus. The protocol outlined here may serve as a reference for future structural determination by cryo-EM of viruses lacking detectable structural homologues.

SUBMITTER: De Colibus L

PROVIDER: S-EPMC5847487 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Building the atomic model of a boreal lake virus of unknown fold in a 3.9 Å cryo-EM map.

De Colibus Luigi L Stuart David I DI

Journal of structural biology 20171027 1

We report here the protocol adopted to build the atomic model of the newly discovered virus FLiP (Flavobacterium infecting, lipid-containing phage) into 3.9 Å cryo-electron microscopy (cryo-EM) maps. In particular, this report discusses the combination of density modification procedures, automatic model building and bioinformatics tools applied to guide the tracing of the major capsid protein (MCP) of this virus. The protocol outlined here may serve as a reference for future structural determina ...[more]

PMID: 29092773

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