Ontology highlight
ABSTRACT:
SUBMITTER: Lupini L
PROVIDER: S-EPMC5847549 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Lupini Laura L Moretti Anna A Bassi Cristian C Schirone Alessio A Pedriali Massimo M Querzoli Patrizia P Roncarati Roberta R Frassoldati Antonio A Negrini Massimo M
Scientific reports 20180312 1
Approximately 70% of breast cancers (BCs) express estrogen receptor alpha (ERα) and are treated with endocrine therapy. However, the effectiveness of this therapy is limited by innate or acquired resistance in approximately one-third of patients. Activating mutations in the ESR1 gene that encodes ERα promote critical resistance mechanisms. Here, we developed a high sensitivity approach based on enhanced-ice-COLD-PCR for detecting ESR1 mutations. The method produced an enrichment up to 100-fold a ...[more]