Dataset Information

Improved water use efficiency and shorter life cycle of Nicotiana tabacum due to modification of guard and vascular companion cells.

ABSTRACT: Severe droughts are predicted for the twenty-first century, which contrast with the increased demand for plant materials. Thus, to sustain future generations, a great challenge is to improve crop yield and water use efficiency (WUE), which is the carbon gained per water lost. Here, expression of maize NADP-malic enzyme (NADP-ME) in the guard and vascular companion cells of Nicotiana tabacum results in enhanced WUE, earlier flowering and shorter life cycle. Transgenic lines exhibit reduced stomatal aperture than wild-type (WT). Nevertheless, an increased net CO2 fixation rate is observed, which results in less water consumption and more biomass production per water used. Transgenic lines export sugars to the phloem at higher rate than WT, which leads to higher sugars levels in phloem exudates and veins. Leaf quantitative proteomic profiling revealed drastic differences in proteins related to cell cycle, flowering, hormone signaling and carbon metabolism between transgenic lines and WT. We propose that the increased sugar export from leaves in the transgenic lines alleviates sugar negative feedback on photosynthesis and thus, stomatal closure takes place without a penalty in CO2 assimilation rate. This results in improved WUE and accelerated overall life cycle, key traits for plant productivity in the near future world.

SUBMITTER: Muller GL

PROVIDER: S-EPMC5847574 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Improved water use efficiency and shorter life cycle of Nicotiana tabacum due to modification of guard and vascular companion cells.

Müller Gabriela L GL Lara María V MV Oitaven Pablo P Andreo Carlos S CS Maurino Verónica G VG Drincovich María F MF

Scientific reports 20180312 1

Severe droughts are predicted for the twenty-first century, which contrast with the increased demand for plant materials. Thus, to sustain future generations, a great challenge is to improve crop yield and water use efficiency (WUE), which is the carbon gained per water lost. Here, expression of maize NADP-malic enzyme (NADP-ME) in the guard and vascular companion cells of Nicotiana tabacum results in enhanced WUE, earlier flowering and shorter life cycle. Transgenic lines exhibit reduced stomat ...[more]

PMID: 29531244

Similar Datasets

Project description:Pollen, an extremely reduced bicellular or tricellular male reproductive structure of flowering plants, serves as a model for numerous studies covering wide range of developmental and physiological processes. The pollen development represents a fragile and vital phase of plant ontogenesis and pollen was among the first singular plant tissues thoroughly characterized at the transcriptomic level (Honys and Twell [5]). Arabidopsis pollen developmental transcriptome has been published over a decade ago (Honys and Twell, 2004) and transcriptomes of developing pollen of other species have followed (Rice, Deveshwar et al. [2]; Triticeae, Tran et al. [11]; upland cotton, Ma et al. [8]). However, the transcriptomic data describing the development of tobacco pollen, a bicellular model for cell biology studies, have been missing. Here we provide the transcriptomic data covering three stages (Tupý et al., 1983) of wild type tobacco (Nicotiana tabacum, cv. Samsun) pollen development: uninucleate microspores (UNM, stage 1), early bicellular pollen (eBCP, stage 3) and late bicellular pollen (lBCP, stage 5) as a supplement to the mature pollen (MP), 4 h-pollen tube (PT4), 24 h-pollen tubes (PT24), leaf (LF) and root (RT) transcriptomic data presented in our previous studies (Hafidh et al., 2012a; Hafidh et al., 2012b). We characterized these transcriptomes to refine the knowledge base of male gametophyte-enriched genes as well as genes expressed preferentially at the individual stages of pollen development. Alongside updating the list of tissue-specific genes, we have investigated differentially expressed genes with respect to early expressed genes. Pollen tube growth and competition of pollen tubes in female pistil can be viewed as a race of the fittest. Accordingly, there is an apparent evolutionary trend among higher plants to store significant material reserves and nutrients during pollen maturation. This supply ensures that after pollen germination, the pollen tube utilizes its resource predominantly for its rapid elongation in the female pistil. Previous transcriptomic data from Arabidopsis showed massive expression of genes encoding proteins forming both ribosomal subunits that were accumulated in developing pollen, whereas their expression was not detectable in growing pollen tubes (Honys and Twell, 2004). We observed a similar phenomenon in less advanced bicellular tobacco pollen. Here, we describe in detail how we obtained and analyzed validated microarray dataset deposited in Gene Expression Omnibus (GSE62349).

Project description:CP12 is a small nuclear encoded chloroplast protein that has been shown to form a complex with the Calvin cycle enzymes, PRK and GADPH. We have taken an antisense approach in order to address the importance of this regulatory protein on carbon assimilation. Transgenic Nicotiana tabacum cv. Samsun with reduced levels of the CP12 protein, produced by leaf disc transformation with a full length tobacco CP12 cDNA, regulated by the cauliflower mosaic virus 35S promoter. Agrobacterium tumefaciens-mediated transformation and selection on kanamycin produced primary transformants whose progeny segregate for severity of the antisense CP12 phenotype. Because of the impact of the antisense reductions in CP12 on carbon assimilation, these plants have abnormal morphology of leaves, floral organs, greatly reduced fertility and markedly slower growth rates. Our hypothesis is that the phenotype is exposing the regulatory nature of the CP12 protein. Intermediates leaving the Calvin cycle are essential for the synthesis of hormones and perturbation of hormone balances is a potential source of the morphological and development effects observed in the CP12 antisense plants. Our objective is to understand the CP12 phenotype by identifying changes in gene expression occurring at key stages in the life cycle of the CP12 antisense plants. CP12 antisense and wild type control plants for microarray analysis were germinated in a growth chamber in agar medium with 1% sucrose and 1/2 strength MS; with a 16 h light/8 h dark cycle at 25°C. At 20 days plants were transferred to a controlled environment greenhouse and grown in Fisons Levington F2 compost at 25°C, with light levels above 500 umol m-2 s-1 ;using supplementary lighting in a 16 h light/8 h dark cycle. 3 independent sets of plants (both wild type and CP12 antisense) were grown for preparation of three independent biological replicate RNA samples. RNA was extracted according to the TIGR protocol from plants in the early in all samples. Keywords: Direct comparison

Dataset Information

Improved water use efficiency and shorter life cycle of Nicotiana tabacum due to modification of guard and vascular companion cells.

Publications

Improved water use efficiency and shorter life cycle of Nicotiana tabacum due to modification of guard and vascular companion cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets