Dataset Information

The dynamics of TGF-? in dental pulp, odontoblasts and dentin.

ABSTRACT: Transforming growth factor-beta (TGF-?) is critical for cell proliferation and differentiation in dental pulp. Here, we show the dynamic mechanisms of TGF-? in porcine dental pulp, odontoblasts and dentin. The mRNA of latent TGF-?1 and TGF-?3 is predominantly expressed in odontoblasts, whereas the mRNA expression level of latent TGF-?2 is high in dental pulp. TGF-?1 is a major isoform of TGF-?, and latent TGF-?1, synthesized in dental pulp, is primarily activated by matrix metalloproteinase 11 (MMP11). Activated TGF-?1 enhances the mRNA expression levels of MMP20 and full-length dentin sialophosphoprotein (DSPP) in dental pulp cells, coinciding with the induction of odontoblast differentiation. Latent TGF-?1 synthesized in odontoblasts is primarily activated by MMP2 and MMP20 in both odontoblasts and dentin. The activity level of TGF-?1 was reduced in the dentin of MMP20 null mice, although the amount of latent TGF-?1 expression did not change between wild-type and MMP20 null mice. TGF-?1 activity was reduced with the degradation of DSPP-derived proteins that occurs with ageing. We propose that to exert its multiple biological functions, TGF-?1 is involved in a complicated dynamic interaction with matrix metalloproteinases (MMPs) and/or DSPP-derived proteins present in dental pulp, odontoblasts and dentin.

SUBMITTER: Niwa T

PROVIDER: S-EPMC5849713 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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The dynamics of TGF-β in dental pulp, odontoblasts and dentin.

Niwa Takahiko T Yamakoshi Yasuo Y Yamazaki Hajime H Karakida Takeo T Chiba Risako R Hu Jan C-C JC Nagano Takatoshi T Yamamoto Ryuji R Simmer James P JP Margolis Henry C HC Gomi Kazuhiro K

Scientific reports 20180313 1

Transforming growth factor-beta (TGF-β) is critical for cell proliferation and differentiation in dental pulp. Here, we show the dynamic mechanisms of TGF-β in porcine dental pulp, odontoblasts and dentin. The mRNA of latent TGF-β1 and TGF-β3 is predominantly expressed in odontoblasts, whereas the mRNA expression level of latent TGF-β2 is high in dental pulp. TGF-β1 is a major isoform of TGF-β, and latent TGF-β1, synthesized in dental pulp, is primarily activated by matrix metalloproteinase 11 ( ...[more]

PMID: 29535349

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Dataset Information

The dynamics of TGF-? in dental pulp, odontoblasts and dentin.

Publications

The dynamics of TGF-β in dental pulp, odontoblasts and dentin.

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