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High-throughput fecundity measurements in Drosophila.

ABSTRACT: Fecundity is probably the most frequently studied fitness component in Drosophila. Nevertheless, currently used methods to measure fecundity are not well-suited for large-scale experiments, with many populations being assayed in parallel. Here we present a standardized pipeline to measure fecundity in many Drosophila population samples with substantially reduced hand on times. Using a high-contrast medium for egg laying, we developed a Java plug-in for ImageJ to quantify the number of eggs by image processing. We show that our method is fast and provides reliable egg counts.

SUBMITTER: Nouhaud P

PROVIDER: S-EPMC5849729 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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High-throughput fecundity measurements in Drosophila.

Nouhaud Pierre P Mallard François F Poupardin Rodolphe R Barghi Neda N Schlötterer Christian C

Scientific reports 20180313 1

Fecundity is probably the most frequently studied fitness component in Drosophila. Nevertheless, currently used methods to measure fecundity are not well-suited for large-scale experiments, with many populations being assayed in parallel. Here we present a standardized pipeline to measure fecundity in many Drosophila population samples with substantially reduced hand on times. Using a high-contrast medium for egg laying, we developed a Java plug-in for ImageJ to quantify the number of eggs by im ...[more]

PMID: 29535355

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