Ontology highlight
ABSTRACT:
SUBMITTER: Qazi SJS
PROVIDER: S-EPMC5852267 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Qazi S Junaid S SJS Turner Raymond J RJ
Biochemistry and biophysics reports 20180220
EmrE is a member of the small multidrug resistance (SMR) protein family in <i>Escherichia coli</i>. It confers resistance to a wide variety of quaternary cation compounds (QCCs) as an efflux transporter driven by the transmembrane proton motive force. We have expressed hexahistidinyl (His<sub>6</sub>) - myc epitope tagged EmrE, extracted it from membrane preparations using the detergent n-dodecyl-β-D-maltopyranoside (DDM), and purified it using nickel-affinity chromatography. The size of the Emr ...[more]