An Ultrasound Assessed Extraction Combined with Ion-Pair HPLC Method and Risk Assessment of Nitrite and Nitrate in Cured Meat.

ABSTRACT: An accurate IPC-UV method was developed and validated for the determination of nitrite (NI) and nitrate (NA) in meat products. The best separation was achieved on a phenyl-hexyl column (150?mm?×?4.6?mm, 3?µm) with a mobile phase composed of 25% acetonitrile and 75% buffer (2?mM disodium hydrogen phosphate and 3?mM tetrabutylammonium bromide, pH?=?4). Eluents were monitored at 205?nm. Linearity ranges were 1.86?×?10-6-7.5?µg·ml-1 and 0.09-5.0?µg·ml-1 for NI and NA, respectively. The correlation coefficients were greater than 0.999 for NI and NA. This method was applied to a number of processed meat products in Riyadh (n?=?155). NI ranged from 1.78 to 129.69?mg·kg-1, and NA ranged from 0.76 to 96.64?mg·kg-1. Results showed extensive use of NI and NA; however, concentrations were within the legal limit of Saudi Arabia except for one sample. Further, the risk assessment and dietary exposure have been estimated for both NI and NA.

SUBMITTER: Abdulkair BY 

PROVIDER: S-EPMC5852839 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature