Staphylococcus aureus CidC Is a Pyruvate:Menaquinone Oxidoreductase.

ABSTRACT: Recent studies have revealed an important role for the Staphylococcus aureus CidC enzyme in cell death during the stationary phase and in biofilm development and have contributed to our understanding of the metabolic processes that are important in the induction of bacterial programmed cell death (PCD). To gain more insight into the characteristics of this enzyme, we performed an in-depth biochemical and biophysical analysis of its catalytic properties. In vitro experiments show that this flavoprotein catalyzes the oxidative decarboxylation of pyruvate to acetate and carbon dioxide. CidC efficiently reduces menadione, but not CoenzymeQ₀, suggesting a specific role in the S. aureus respiratory chain. CidC exists as a monomer under neutral-pH conditions but tends to aggregate and

SUBMITTER: Zhang X 

PROVIDER: S-EPMC5853648 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature