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Light-triggered enzymatic reactions in nested vesicle reactors.


ABSTRACT: Cell-sized vesicles have tremendous potential both as miniaturised pL reaction vessels and in bottom-up synthetic biology as chassis for artificial cells. In both these areas the introduction of light-responsive modules affords increased functionality, for example, to initiate enzymatic reactions in the vesicle interior with spatiotemporal control. Here we report a system composed of nested vesicles where the inner compartments act as phototransducers, responding to ultraviolet irradiation through diacetylene polymerisation-induced pore formation to initiate enzymatic reactions. The controlled release and hydrolysis of a fluorogenic ?-galactosidase substrate in the external compartment is demonstrated, where the rate of reaction can be modulated by varying ultraviolet exposure time. Such cell-like nested microreactor structures could be utilised in fields from biocatalysis through to drug delivery.

SUBMITTER: Hindley JW 

PROVIDER: S-EPMC5854585 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Light-triggered enzymatic reactions in nested vesicle reactors.

Hindley James W JW   Elani Yuval Y   McGilvery Catriona M CM   Ali Simak S   Bevan Charlotte L CL   Law Robert V RV   Ces Oscar O  

Nature communications 20180315 1


Cell-sized vesicles have tremendous potential both as miniaturised pL reaction vessels and in bottom-up synthetic biology as chassis for artificial cells. In both these areas the introduction of light-responsive modules affords increased functionality, for example, to initiate enzymatic reactions in the vesicle interior with spatiotemporal control. Here we report a system composed of nested vesicles where the inner compartments act as phototransducers, responding to ultraviolet irradiation throu  ...[more]

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