Ontology highlight
ABSTRACT:
SUBMITTER: Ling AK
PROVIDER: S-EPMC5856553 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Ling Alexanda K AK So Clare C CC Le Michael X MX Chen Audrey Y AY Hung Lisa L Martin Alberto A
Proceedings of the National Academy of Sciences of the United States of America 20180222 11
Activation-induced cytidine deaminase (AID) inflicts DNA damage at Ig genes to initiate class switch recombination (CSR) and chromosomal translocations. However, the DNA lesions formed during these processes retain an element of randomness, and thus knowledge of the relationship between specific DNA lesions and AID-mediated processes remains incomplete. To identify necessary and sufficient DNA lesions in CSR, the Cas9 endonuclease and nickase variants were used to program DNA lesions at a greate ...[more]