Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins.

ABSTRACT: Here, we report the site-specific incorporation of a thioester containing noncanonical amino acid (ncAA) into recombinantly expressed proteins. Specifically, we genetically encoded a thioester-activated aspartic acid (ThioD) in bacteria in good yield and with high fidelity using an orthogonal nonsense suppressor tRNA/aminoacyl-tRNA synthetase (aaRS) pair. To demonstrate the utility of ThioD, we used native chemical ligation to label green fluorescent protein with a fluorophore in good yield.

SUBMITTER: Xuan W 

PROVIDER: S-EPMC5856652 | biostudies-literature |

REPOSITORIES: biostudies-literature