Dataset Information

Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins.

ABSTRACT: Here, we report the site-specific incorporation of a thioester containing noncanonical amino acid (ncAA) into recombinantly expressed proteins. Specifically, we genetically encoded a thioester-activated aspartic acid (ThioD) in bacteria in good yield and with high fidelity using an orthogonal nonsense suppressor tRNA/aminoacyl-tRNA synthetase (aaRS) pair. To demonstrate the utility of ThioD, we used native chemical ligation to label green fluorescent protein with a fluorophore in good yield.

SUBMITTER: Xuan W

PROVIDER: S-EPMC5856652 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins.

Xuan Weimin W Collins Daniel D Koh Minseob M Shao Sida S Yao Anzhi A Xiao Han H Garner Philip P Schultz Peter G PG

ACS chemical biology 20180209 3

Here, we report the site-specific incorporation of a thioester containing noncanonical amino acid (ncAA) into recombinantly expressed proteins. Specifically, we genetically encoded a thioester-activated aspartic acid (ThioD) in bacteria in good yield and with high fidelity using an orthogonal nonsense suppressor tRNA/aminoacyl-tRNA synthetase (aaRS) pair. To demonstrate the utility of ThioD, we used native chemical ligation to label green fluorescent protein with a fluorophore in good yield. ...[more]

PMID: 29360343

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Dataset Information

Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins.

Publications

Site-Specific Incorporation of a Thioester Containing Amino Acid into Proteins.

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