Ontology highlight
ABSTRACT:
SUBMITTER: Kornete M
PROVIDER: S-EPMC5857648 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Kornete Mara M Marone Romina R Jeker Lukas T LT
Journal of immunology (Baltimore, Md. : 1950) 20180214 7
Adoptive cell transfer is an important approach for basic research and emerges as an effective treatment for various diseases, including infections and blood cancers. Direct genetic manipulation of primary immune cells opens up unprecedented research opportunities and could be applied to enhance cellular therapeutic products. In this article, we report highly efficient genome engineering in primary murine T cells using a plasmid-based RNA-guided CRISPR system. We developed a straightforward appr ...[more]