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RNA interference is essential for cellular quiescence.


ABSTRACT: Quiescent cells play a predominant role in most organisms. Here we identify RNA interference (RNAi) as a major requirement for quiescence (G0 phase of the cell cycle) in Schizosaccharomyces pombe RNAi mutants lose viability at G0 entry and are unable to maintain long-term quiescence. We identified suppressors of G0 defects in cells lacking Dicer (dcr1?), which mapped to genes involved in chromosome segregation, RNA polymerase-associated factors, and heterochromatin formation. We propose a model in which RNAi promotes the release of RNA polymerase in cycling and quiescent cells: (i) RNA polymerase II release mediates heterochromatin formation at centromeres, allowing proper chromosome segregation during mitotic growth and G0 entry, and (ii) RNA polymerase I release prevents heterochromatin formation at ribosomal DNA during quiescence maintenance. Our model may account for the codependency of RNAi and histone H3 lysine 9 methylation throughout eukaryotic evolution.

SUBMITTER: Roche B 

PROVIDER: S-EPMC5858868 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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RNA interference is essential for cellular quiescence.

Roche B B   Arcangioli B B   Martienssen R A RA  

Science (New York, N.Y.) 20161013 6313


Quiescent cells play a predominant role in most organisms. Here we identify RNA interference (RNAi) as a major requirement for quiescence (G<sub>0</sub> phase of the cell cycle) in Schizosaccharomyces pombe RNAi mutants lose viability at G<sub>0</sub> entry and are unable to maintain long-term quiescence. We identified suppressors of G<sub>0</sub> defects in cells lacking Dicer (dcr1Δ), which mapped to genes involved in chromosome segregation, RNA polymerase-associated factors, and heterochromat  ...[more]

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