Ontology highlight
ABSTRACT:
SUBMITTER: Wang H
PROVIDER: S-EPMC5858877 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Wang Huaimin H Feng Zhaoqianqi Z Del Signore Steven J SJ Rodal Avital A AA Xu Bing B
Journal of the American Chemical Society 20180301 10
Despite the advancement of molecular imaging techniques, there is an unmet need for probes for direct imaging of membrane dynamics of live cells. Here we report a novel type of active (or enzyme responsive) probes to directly image membrane dynamics of live cells with high spatial and temporal resolution over extended time scales and areas. Because lipid rafts enrich cholesterols and GPI-anchored enzymes (e.g., ectophosphatases), we design probes that consist of an enzymatic trigger, a fluoropho ...[more]