Ontology highlight
ABSTRACT:
SUBMITTER: Preissl S
PROVIDER: S-EPMC5862073 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Preissl Sebastian S Fang Rongxin R Huang Hui H Zhao Yuan Y Raviram Ramya R Gorkin David U DU Zhang Yanxiao Y Sos Brandon C BC Afzal Veena V Dickel Diane E DE Kuan Samantha S Visel Axel A Pennacchio Len A LA Zhang Kun K Ren Bing B
Nature neuroscience 20180212 3
Analysis of chromatin accessibility can reveal transcriptional regulatory sequences, but heterogeneity of primary tissues poses a significant challenge in mapping the precise chromatin landscape in specific cell types. Here we report single-nucleus ATAC-seq, a combinatorial barcoding-assisted single-cell assay for transposase-accessible chromatin that is optimized for use on flash-frozen primary tissue samples. We apply this technique to the mouse forebrain through eight developmental stages. Th ...[more]