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Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors.

ABSTRACT: The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.

SUBMITTER: Jain PK

PROVIDER: S-EPMC5864249 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors.

Jain Piyush K PK Ramanan Vyas V Schepers Arnout G AG Dalvie Nisha S NS Panda Apekshya A Fleming Heather E HE Bhatia Sangeeta N SN

Angewandte Chemie (International ed. in English) 20160824 40

The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeli ...[more]

PMID: 27554600

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