Optimizing "self-wicking" nanowire grids.
Ontology highlight
ABSTRACT:
SUBMITTER: Wei H
PROVIDER: S-EPMC5864531 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Publications
Optimizing "self-wicking" nanowire grids.
Wei Hui H Dandey Venkata P VP Zhang Zhening Z Raczkowski Ashleigh A Rice Willam J WJ Carragher Bridget B Potter Clinton S CS
Journal of structural biology 20180106 2
We have developed a self-blotting TEM grid for use with a novel instrument for vitrifying samples for cryo-electron microscopy (cryoEM). Nanowires are grown on the copper surface of the grid using a simple chemical reaction and the opposite smooth side is used to adhere to a holey sample substrate support, for example carbon or gold. When small volumes of sample are applied to the nanowire grids the wires effectively act as blotting paper to rapidly wick away the liquid, leaving behind a thin fi ...[more]
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