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The effects of hydroxyethyl starch and gelatine on pulmonary cytokine production and oedema formation.


ABSTRACT: Recently, side effects of plasma expanders like hydroxyethyl starch and gelatine gained considerable attention. Most studies have focused on the kidneys; lungs remain unconsidered. Isolated mouse lungs were perfused for 4?hours with buffer solutions based on hydroxyethyl starch (HES) 130/0.4, HES 200/0.5 or gelatine and ventilated with low or high pressure under physiological pH and alkalosis. Outcome parameters were cytokine levels and the wet-to-dry ratio. For cytokine release, murine and human PCLS were incubated in three different buffers and time points.In lungs perfused with the gelatine based buffer IL-6, MIP-2 and KC increased when ventilated with high pressure. Wet-to-dry ratios increased stronger in lungs perfused with gelatine - compared to HES 130/0.4. Alkalotic perfusion resulted in higher cytokine levels but normal wet-to-dry ratio. Murine PCLS supernatants showed increased IL-6 and KC when incubated in gelatine based buffer, whereas in human PCLS IL-8 was elevated. In murine IPL HES 130/0.4 has lung protective effects in comparison to gelatine based infusion solutions, especially in the presence of high-pressure ventilation. Gelatine perfusion resulted in increased cytokine production. Our findings suggest that gelatine based solutions may have side effects in patients with lung injury or lung oedema.

SUBMITTER: Krabbe J 

PROVIDER: S-EPMC5865122 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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The effects of hydroxyethyl starch and gelatine on pulmonary cytokine production and oedema formation.

Krabbe Julia J   Ruske Nadine N   Braunschweig Till T   Kintsler Svetlana S   Spillner Jan W JW   Schröder Thomas T   Kalverkamp Sebastian S   Kanzler Stephanie S   Rieg Annette D AD   Uhlig Stefan S   Martin Christian C  

Scientific reports 20180323 1


Recently, side effects of plasma expanders like hydroxyethyl starch and gelatine gained considerable attention. Most studies have focused on the kidneys; lungs remain unconsidered. Isolated mouse lungs were perfused for 4 hours with buffer solutions based on hydroxyethyl starch (HES) 130/0.4, HES 200/0.5 or gelatine and ventilated with low or high pressure under physiological pH and alkalosis. Outcome parameters were cytokine levels and the wet-to-dry ratio. For cytokine release, murine and huma  ...[more]

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