Ontology highlight
ABSTRACT:
SUBMITTER: Tyler DS
PROVIDER: S-EPMC5865750 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20170615 6345
The success of new therapies hinges on our ability to understand their molecular and cellular mechanisms of action. We modified BET bromodomain inhibitors, an epigenetic-based therapy, to create functionally conserved compounds that are amenable to click chemistry and can be used as molecular probes in vitro and in vivo. We used click proteomics and click sequencing to explore the gene regulatory function of BRD4 (bromodomain containing protein 4) and the transcriptional changes induced by BET i ...[more]