Expression of immunoglobulin A in human mesangial cells and its effects on cell apoptosis and adhesion.

ABSTRACT: IgA nephropathy (IgAN) is characterized by predominant IgA deposition in the glomerular mesangium. It has been considered that the deposited IgA is synthesized by B cells, although recent reports have suggested the implication of other cell types. Therefore, the present study investigated whether glomerular mesangial cells could produce IgA by themselves. Semi?quantitative reverse transcription-polymerase chain reaction, and immunostaining analysis revealed that the IgA protein and gene transcripts were expressed in primary human renal mesangial cells (HRMCs). Furthermore, the IgA heavy chain (?1 and ?2) and the light chain (? and ?) were localized in the cytoplasm or were located on the cell membranes of human mesangial cells (HMCs). Mass spectrometry results indicated that Ig ?1 and Ig ?2 were secreted in the culture media of HMCs. The transcripts of Ig ?, Ig ? and Ig ? constant regions were detected. The predominant rearrangement pattern of the variable region of Ig ?, was V?3?20*01/J?1*01 in HMCs and V?1?12*01/J?4*01 in HRMCs. In addition, knockdown of Ig ?1 expression by small interfering RNA (siRNA) inhibited cell adhesion and promoted apoptosis. Our findings demonstrate that HMCs can express IgA, and that this expression is associated with cell functions, which may contribute to the deposition of IgA in patients with IgAN.

SUBMITTER: Deng H 

PROVIDER: S-EPMC5865995 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature