Ontology highlight
ABSTRACT:
SUBMITTER: Diao J
PROVIDER: S-EPMC5868476 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Frontiers in microbiology 20180319
In this study, we evaluated suitable selected markers and optimized transformation protocols to develop a new genetic transformation methodology for DHA-producing <i>Crypthecodinium cohnii</i>. Additionally, ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), potentially involved in CO<sub>2</sub> fixation under autotrophic conditions, was selected as the target for construction of a gene knockdown mutant. Our results show that the constructs were successfully inserted into the <i>C. cohn ...[more]