Dataset Information

How much is too much?-Influence of X-ray dose on root growth of faba bean (Vicia faba) and barley (Hordeum vulgare).

ABSTRACT: X-ray CT is a powerful technology to study root growth in soil in-situ. Root systems can be studied in its true 3D geometry over time. Hence, the same plant can be scanned multiple times during development. A downside is the potential of X-rays to interfere with biological processes and therefore plant growth. The aim of this study is to evaluate the influence of cumulative X-ray dose on Vicia faba and Hordeum vulgare during a growth period of 17 days. One control treatment without X-ray scanning was compared to two treatments being scanned every two and four days, respectively. Scanned treatments received a maximum cumulative dose of less than 8 Gy. Plant species differed in their susceptibility to X-ray dose. For Vicia faba, mean total root length was reduced significantly. Leave growth was reduced as well. Number and length of second order laterals was reduced significantly, as well as length of first order laterals. Hordeum vulgare showed no negative impact of X-ray dose on any of the root parameters. Large differences between the two species investigated were detected in respect to susceptibility to X-ray dose. Results indicate that for X-ray CT studies involving temporal resolution a control treatment without scanning is required.

SUBMITTER: Blaser SRGA

PROVIDER: S-EPMC5868774 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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How much is too much?-Influence of X-ray dose on root growth of faba bean (Vicia faba) and barley (Hordeum vulgare).

Blaser Sebastian R G A SRGA Schlüter Steffen S Vetterlein Doris D

PloS one 20180326 3

X-ray CT is a powerful technology to study root growth in soil in-situ. Root systems can be studied in its true 3D geometry over time. Hence, the same plant can be scanned multiple times during development. A downside is the potential of X-rays to interfere with biological processes and therefore plant growth. The aim of this study is to evaluate the influence of cumulative X-ray dose on Vicia faba and Hordeum vulgare during a growth period of 17 days. One control treatment without X-ray scannin ...[more]

PMID: 29579093

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Project description:BackgroundSoil moisture deficiency causes yield reduction and instability in faba bean (Vicia faba L.) production. The extent of sensitivity to drought stress varies across accessions originating from diverse moisture regimes of the world. Hence, we conducted successive greenhouse experiments in pots and rhizotrons to explore diversity in root responses to soil water deficit.MethodsA set of 89 accessions from wet and dry growing regions of the world was defined according to the Focused Identification of Germplasm Strategy and screened in a perlite-sand medium under well watered conditions in a greenhouse experiment. Stomatal conductance, canopy temperature, chlorophyll concentration, and root and shoot dry weights were recorded during the fifth week of growth. Eight accessions representing the range of responses were selected for further investigation. Starting five days after germination, they were subjected to a root phenotyping experiment using the automated phenotyping platform GROWSCREEN-Rhizo. The rhizotrons were filled with peat-soil under well watered and water limited conditions. Root architectural traits were recorded five, 12, and 19 days after the treatment (DAT) began.ResultsIn the germplasm survey, accessions from dry regions showed significantly higher values of chlorophyll concentration, shoot and root dry weights than those from wet regions. Root and shoot dry weight as well as seed weight, and chlorophyll concentration were positively correlated with each other. Accession DS70622 combined higher values of root and shoot dry weight than the rest. The experiment in GROWSCREEN-Rhizo showed large differences in root response to water deficit. The accession by treatment interactions in taproot and second order lateral root lengths were significant at 12 and 19 DAT, and the taproot length was reduced up to 57% by drought. The longest and deepest root systems under both treatment conditions were recorded by DS70622 and DS11320, and total root length of DS70622 was three times longer than that of WS99501, the shortest rooted accession. The maximum horizontal distribution of a root system and root surface coverage were positively correlated with taproot and total root lengths and root system depth. DS70622 and WS99501 combined maximum and minimum values of these traits, respectively. Thus, roots of DS70622 and DS11320, from dry regions, showed drought-avoidance characteristics whereas those of WS99501 and Mèlodie/2, from wet regions, showed the opposite.DiscussionThe combination of the germplasm survey and use of GROWSCREEN-Rhizo allowed exploring of adaptive traits and detection of root phenotypic markers for potential drought avoidance. The greater root system depth and root surface coverage, exemplified by DS70622 and DS11320, can now be tested as new sources of drought tolerance.

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Dataset Information

How much is too much?-Influence of X-ray dose on root growth of faba bean (Vicia faba) and barley (Hordeum vulgare).

Publications

How much is too much?-Influence of X-ray dose on root growth of faba bean (Vicia faba) and barley (Hordeum vulgare).

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