Ontology highlight
ABSTRACT:
SUBMITTER: Alper BJ
PROVIDER: S-EPMC5869690 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Alper Benjamin J BJ Rowse Jarrad W JW Schmidt Walter K WK
Yeast (Chichester, England) 20091101 11
The S. cerevisiae genome encodes two M16A enzymes: Axl1p and Ste23p. Of the two, Ste23p shares significantly higher sequence identity with M16A enzymes from other species, including mammalian insulin-degrading enzymes (IDEs). In this study, recombinant Ste23p and R. norvegicus IDE (RnIDE) were isolated from E. coli, and their enzymatic properties compared. Ste23p was found to cleave established RnIDE substrates, including the amyloid-beta peptide (Abeta1-40) and insulin B-chain. A novel internal ...[more]