Dataset Information

CRISPRcloud: a secure cloud-based pipeline for CRISPR pooled screen deconvolution.

ABSTRACT:

Summary

We present a user-friendly, cloud-based, data analysis pipeline for the deconvolution of pooled screening data. This tool, CRISPRcloud, serves a dual purpose of extracting, clustering and analyzing raw next generation sequencing files derived from pooled screening experiments while at the same time presenting them in a user-friendly way on a secure web-based platform. Moreover, CRISPRcloud serves as a useful web-based analysis pipeline for reanalysis of pooled CRISPR screening datasets. Taken together, the framework described in this study is expected to accelerate development of web-based bioinformatics tool for handling all studies which include next generation sequencing data.

Availability and implementation

http://crispr.nrihub.org.

Contact

zhandong.liu@bcm.edu.

Supplementary information

Supplementary data are available at Bioinformatics online.

SUBMITTER: Jeong HH

PROVIDER: S-EPMC5870715 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

CRISPRcloud: a secure cloud-based pipeline for CRISPR pooled screen deconvolution.

Jeong Hyun-Hwan HH Kim Seon Young SY Rousseaux Maxime W C MWC Zoghbi Huda Y HY Liu Zhandong Z

Bioinformatics (Oxford, England) 20170901 18

<h4>Summary</h4>We present a user-friendly, cloud-based, data analysis pipeline for the deconvolution of pooled screening data. This tool, CRISPRcloud, serves a dual purpose of extracting, clustering and analyzing raw next generation sequencing files derived from pooled screening experiments while at the same time presenting them in a user-friendly way on a secure web-based platform. Moreover, CRISPRcloud serves as a useful web-based analysis pipeline for reanalysis of pooled CRISPR screening da ...[more]

PMID: 28541456

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Dataset Information

CRISPRcloud: a secure cloud-based pipeline for CRISPR pooled screen deconvolution.

Summary

Availability and implementation

Contact

Supplementary information

Publications

CRISPRcloud: a secure cloud-based pipeline for CRISPR pooled screen deconvolution.

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