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CRISPRcloud: a secure cloud-based pipeline for CRISPR pooled screen deconvolution.


ABSTRACT: We present a user-friendly, cloud-based, data analysis pipeline for the deconvolution of pooled screening data. This tool, CRISPRcloud, serves a dual purpose of extracting, clustering and analyzing raw next generation sequencing files derived from pooled screening experiments while at the same time presenting them in a user-friendly way on a secure web-based platform. Moreover, CRISPRcloud serves as a useful web-based analysis pipeline for reanalysis of pooled CRISPR screening datasets. Taken together, the framework described in this study is expected to accelerate development of web-based bioinformatics tool for handling all studies which include next generation sequencing data.http://crispr.nrihub.org.zhandong.liu@bcm.edu.Supplementary data are available at Bioinformatics online.

SUBMITTER: Jeong HH 

PROVIDER: S-EPMC5870715 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature

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CRISPRcloud: a secure cloud-based pipeline for CRISPR pooled screen deconvolution.

Jeong Hyun-Hwan HH   Kim Seon Young SY   Rousseaux Maxime W C MWC   Zoghbi Huda Y HY   Liu Zhandong Z  

Bioinformatics (Oxford, England) 20170901 18


<h4>Summary</h4>We present a user-friendly, cloud-based, data analysis pipeline for the deconvolution of pooled screening data. This tool, CRISPRcloud, serves a dual purpose of extracting, clustering and analyzing raw next generation sequencing files derived from pooled screening experiments while at the same time presenting them in a user-friendly way on a secure web-based platform. Moreover, CRISPRcloud serves as a useful web-based analysis pipeline for reanalysis of pooled CRISPR screening da  ...[more]

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