New targeted approaches for the quantification of data-independent acquisition mass spectrometry.

ABSTRACT: The use of data-independent acquisition (DIA) approaches for the reproducible and precise quantification of complex protein samples has increased in the last years. The protein information arising from DIA analysis is stored in digital protein maps (DIA maps) that can be interrogated in a targeted way by using ad hoc or publically available peptide spectral libraries generated on the same sample species as for the generation of the DIA maps. The restricted availability of certain difficult-to-obtain human tissues (i.e., brain) together with the caveats of using spectral libraries generated under variable experimental conditions limits the potential of DIA. Therefore, DIA workflows would benefit from high-quality and extended spectral libraries that could be generated without the need of us

SUBMITTER: Bruderer R 

PROVIDER: S-EPMC5870755 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature