Ontology highlight
ABSTRACT:
SUBMITTER: Lutz S
PROVIDER: S-EPMC58715 | biostudies-literature | 2001 Sep
REPOSITORIES: biostudies-literature
Lutz S S Ostermeier M M Moore G L GL Maranas C D CD Benkovic S J SJ
Proceedings of the National Academy of Sciences of the United States of America 20010918 20
We have developed, experimentally implemented, and modeled in silico a methodology named SCRATCHY that enables the combinatorial engineering of target proteins, independent of sequence identity. The approach combines two methods for recombining genes: incremental truncation for the creation of hybrid enzymes and DNA shuffling. First, incremental truncation for the creation of hybrid enzymes is used to create a comprehensive set of fusions between fragments of genes in a DNA homology-independent ...[more]